Abstract

Fluorinated stationary phases have been believed to be unique in their ability to separate paclitaxel and related taxanes because of some speculative specific interaction between the fluorinated moiety and the taxanes. On the other hand, hydrocarbonaceous RP phases were held to be unable to separate all these compounds. Our detailed studies of the effect of eluent composition and column temperature show there is no difference in the separation mechanism on several fluorinated phases and on a regular octyl bonded phase for the 15 standard taxane mixture. Especially on the octyl phase, the xylosyl-taxols show an unexpected increase in k' with increasing temperature, whereas the other taxols behave in the expected fashion. The baseline separation of all 15 taxanes on both the fluorinated phases and the octyl phase requires careful control of eluent composition and also of column temperature. Good column temperature control may be a general requirement in HPLC that has long been neglected. We account for the separation mechanism in terms of changes in the composition of the solvation layer on the stationary phase with changes in temperature and eluent composition.

Brief Bio

Prof. David C. Locke has been in the Chemistry Department of Queens College and of the Graduate School of the City University of New York since 1968. He has supervised the PhD. dissertation research of about 30 students, many of whom now work in New Jersey. He graduated from Lafayette College and did his graduate work at Kansas State University. His research interests are in analytical separations and in environmental chemistry.

Directions

Frog Rock Country Club in Hammonton [420 Boyer Ave] near Kessler Hospital off White Horse Pike.

From the North going south on US 30 (White Horse Pike):
The Kessler Hospital will be on the right side, make a left at the light, then a right at the stop sign, then make a left on to Pleasant Mills Rd [542]. Take the next right at Boyer Ave to the restaurant on the left side.

From the South going North on US 30 (White Horse Pike):
The Kessler Hospital will be on the left side, make a right at the light, then a right at the stop sign, then make a left onto Pleasant Mills Rd [542]. Take the next right at Boyer Ave to the restaurant on the left side.

Cost: $15 members and guests, $10 students

Reservation by Monday Feb12

Donna D'Emilio (856) 256-4855 or 256-4856
Michael Casner (856) 384-7048
Thomas Colacot (856) 384-7185
Alice Sebastian (856) 384-7028