The Speaker:

Dr. Seeholzer obtained his Ph.D. from the University of Pennsylvania where he studied carbon flow in cardiac metabolism using isotope labeling, NMR spectroscopy, and mathematical modeling. His post-doctoral career began at Fox Chase Cancer Center studying protein structure and protein-protein interactions using multi-dimensional NMR spectroscopy. He continued there doing research in mechanistic enzymology. He has taught courses in Biochemistry and Advanced Cell Biology at the University of Pennsylvania and spent one year teaching high school chemistry. In 1996 he returned to Fox Chase Cancer Center to build a program in mass spectrometry and proteomics research. The pursuit of his interest in cell metabolism, protein structure, and mechanistic enzymology continue using mass spectrometry as a primary tool. His current proteomics efforts focus on early detection and risk assessment for breast cancer, on prevention of colorectal cancer, and on development of quantitative methods for proteome analysis.

Recent publications:
(a) G. Moraleda, S. Seeholzer, V. Bichko, R.L. Dunbrack, Jr, J. Otto, J. Taylor, , J. Virol., 1999, 73, 7147--7152.(b) T. J. T. Pinheiro, H. Cheng, S. H. Seeholzer, H. Roder, J. Mol. Biol., 2000, 303, 617–626. (c) C. Hall, D. M. Nelson, X. Ye, K. Baker, J. A. DeCaprio, S. Seeholzer, M. Lipinski, P. D. Adams; Mol. Cell. Biol. 2001, 21, 1854–1865. (d) Pinheiro, T.J., Cheng, H., Seeholzer, S.H. & Roder, H. J. Mol. Biol, 2000. 303:617-26. (e) Petronzelli, F., Riccio, A., Markham, G.D., Seeholzer, S.H., Stoerker, J., Genuardi, M., Yeung, A.T., Matsumoto, Y. and Bellacosa, A. J. Biol. Chem. 275:32422-32429 (2000). (f) Petronzelli, F., Riccio, A., Markham, G.D., Seeholzer, S.H., Genuardi, M., Karbowski, M., Yeung, A.T., Matsumoto, Y. and Bellacosa, A. J. Cell. Physiol. 185:473-480 (2000). (g) Spodik, B., Seeholzer, S., Coleman, T.R. Using Xenopus egg extracts to modify recombinant proteins. In Protein-protein Interactions, edited by E. Golemis and I. Serebriiski. Cold Spring Harbor Laboratory Press, New York, pp. 355-374, 2001. (h) Hall, C., Nelson, D.M., Ye, X., Baker, K., DeCaprio, J.A., Seeholzer, S., Lipinski, M., Adams, P.D. Mol. Cell. Biol. (2001) 21:1854-1865. (i) Adams PD, Seeholzer S, Ohh M. Identification of Associated Proteins by Coimmunoprecipitation. In: Protein-Protein Interactions. Published by Cold spring Harbor Laboratory Press. Edited by: Eric Golemis. pp. 59-74. (2002)

Abstract:

Proteomics research has achieved an important status in broad areas of basic and clinical research. This lecture will introduce the concept of the proteome and then discuss the methods we use for its analysis with a particular emphasis on two dimensional electrophoresis and mass spectrometry. Some basic cancer biology will be presented followed by some details of the process of biomarker discovery for the early detection of breast cancer. Also discussed will be a new model for chemoprevention of colorectal cancer.

Directions:
The September meeting will be held at Five Point Diner, which is located at the intersection of Hwy 41 and State Hwy 47 in Deptford. Please call the restaurant at 856-228-5166 for direction.

Social Hour: 5:30-6:30 PM
Dinner: 6:30 PM
Talk 7:30 PM

Dinner selections:
Roast Sirloin of Beef Vegetables Vanilla Ice Cream
Broiled Spring Chicken Dinner Salad Chocolate Ice Cream
Roast Vermont Turkey Sauteed Peas Rice Pudding
Fried Flounder Cole Slaw Bread Pudding
Chicken Parmigiana Baked Idaho Potato Tapioca Pudding
Chicken Caesar salad Soup du Jour

Cost: $15 members and guests, $10 students
Reservations by Friday, 13 September 2002, noon.
Lori Stephans (856) 256-4500 ext. 3551 or ‘stephans@rowan.edu’
Thomas Colacot (856) 384-7185 or ‘colactj@jmusa.com’
Peter Mlynek (856) 787-0770 or ‘pdmlynek@yahoo.com’
Guenter Niessen (856) 423-6300 or ‘gniessen@emscience.com’

Last Update 8/27/02
Maintained by Robert Newland