Faculty and Research Interests
Michael Henry, PhD
Science Center 320
The goal of my research is to examine the cellular mechanism by which mRNA precursors are processed in the nucleus, using the budding yeast Saccharomyces cerevisiae as a model system. I plan to focus on the role of posttranslational modification in this process. Although significant progress has been made in identifying the proteins required for RNA maturation in both yeast and mammals, the set is not yet complete, and the mechanisms by which they act remain poorly understood. A better understanding of this process not only has implications for oncology but also can provide insights into viral mechanisms for RNA maturation during cell infection.
- Henry, M.F., Mandel D., Routson, V. and Henry, P.A. 2003. The Yeast hnRNP-like protein Hrp1/Nab4 accumulates in the cytoplasm following hyperosmotic stress: a novel Fps1-dependent response. Mol Biol. Cell. 14:3929-3941
- Xu, C., Henry, P.A., Setya S. and Henry M.F. 2003. In vivo analysis of nucleolar proteins modified by the yeast arginine methyltransferase Hmt1/Rmt1p. RNA. 6:746-759
- González, C.L., Ruiz-Echevarría, M.J., Vasudevan, S. W. , Henry, M.F.,and S.W. Peltz. 2000. Mutations in HRP1 Suggest That A Nucleocytoplasmic Cross-Talk Is Involved In Nonsense-Mediated mRNA Decay. Mol Cell. 5:489-99
- Gratzer, S., Beilharz, T., Beddoe, T., Henry, M.F., and T. Lithgow. 2000. The mitochondrial protein targeting suppressor (mts1) mutation maps to the mRNA-binding domain of Npl3p and effects translation on cytoplasmic ribosomes. Mol Microbiol. 35: 1277-85
- Klein, S., Carroll, J.A., Chen, Y., Henry, M.F., Ortonowski, I., Gravotta, D., Pinucci, G., Beavis, R.C., Burgess, W.H. and Rifken, D.B. 2000. Biochemical Analysis of the Methylation of high Molecular Weight Fibroblast Growth Factor-2. J Biol Chem. 275:3150-7
- Scott*, H.S., Antonarakis, S.E., Lalioti, M.D., Rossier,C., Silver, P.A., and M.F. Henry*. 1998. Identification and characterization of two putative human arginine methyltransferases (HRMT1L1 and HRMT1L2). Genomics 48: 330-340
- Shen, E.C., Henry, M.F., Weiss, V.H., Valentini, S.R., Silver, P.A., and Lee, M.S. (1998) Arginine methylation facilitates the nuclear export of hnRNP proteins. Genes Dev. 12: 679-691
- Kessler*, M., Henry*, M.F., Shen E., Gross, S., Silver, P.A., and C. Moore. 1998. HRP1 is a specific RNA binding protein that shuttles between the nucleus and cytoplasm and is required for 3' end formation in yeast. Genes Dev. 11: 2545-2556
- Corbett, A.H., Ferrigno, P., Henry, M.F., Kahana, J., Koepp, D.M., Lee, M.S., Nguyen, L., Schlenstedt, G., Seedorf, M., Shen, E.C., Taura, T., Wong, D.H., and P.A. Silver. 1996. Genetic Analysis of Macromolecular Transport across the Nuclear Envelope. In: Proceedings of the Nobel Symposium on the Nucleus. Exp. Cell Res. 229: 212-216
- Henry, M.F., and P.A. Silver. 1996. A Novel Methyltransferase (HMT1) Modifies Poly(A)+ RNA-Binding Proteins. Mol. Cell. Biol. 16: 3668-3678
- Lee, M.S., M.F. Henry, and P.A. Silver. 1996. A Protein That Shuttles Between the Nucleus and the Cytoplasm is an Important Mediator of RNA Export. Genes Dev. 10: 1233-1246.
- Henry, M.F., C.Z. Borland, M. Bossie, and P.A. Silver. 1996. Potential RNA Binding Proteins in Saccharomyces cerevisiae Identified as Suppressors of Temperature-Sensitive Mutations in NPL3. Genetics 142: 103-115
- Henry M.F., and J.E. Cronan, Jr. 1992. A New Mechanism of Transcriptional Regulation: Release of an Activator Triggered by Small Molecule Binding. Cell 70: 671-679.
- Henry, M.F., and J.E. Cronan, Jr. 1991. Escherichia coli Transcription Factor That Both Activates Fatty Acid Synthesis and Represses Fatty Acid Degradation. J. Mol. Biol. 222:843-849.
- Henry, M.F., and J.E. Cronan, Jr. 1991. A Direct and General Selection for Lysogens of Escherichia coli by Phage Lambda Recombinant Clones. J. Bacteriol. 173:3724-3731.
- Henry, M.F., and J.E. Cronan, Jr. 1989. A Facile and Reversible Method to Decrease the Copy Number of ColE1-Related Cloning Vectors Commonly Used in Escherichia coli. J. Bacteriol. 122:5254-5261.