Collaborative Support Faculty

Benjamin R. Carone

Assistant Professor, Biological Sciences
carone@rowan.edu

Education

BS (Molecular & Cellular Biology, Philosophy), University of Connecticut
PhD (Genetics and Genomics), University of Connecticut
Postdoctoral (Transgenerational Inheritance/Epigenetics), University of Massachusetts Medical School
Visiting Assistant Professor (Histone Modifications), Williams College

Research Expertise

Epigenetics | Genomics | Histone modifications

My research interests are in the field of Epigenetics, which I approach by working to identify the epigenetic marks and molecular mechanisms responsible for causing and maintaining the inheritance of acquired states.

  • Investigating the capacity of conserved protein catalytic domains to establish and maintain epigenetic modifications in S. cerevisiae with the ultimate goal establishing the causal role of histone packaging in regulating gene expression.  Specifically, my laboratory has created a suite of fusion proteins using CRISPR technology to target H3K9me to eGFP tagged endogenous S. cerevisiae genes which we interrogate expression levels using qRT-PCR as flow cytometry.

Testing the hypothesis that mammalian spermatic chromatin is highly organized and that this patterning can function to drive Transgenerational Epigenetic Inheritance.  Determining the genome-wide organization of chromatin in germ cells using genomic and bioinformatics approaches.  We are currently working with previously/externally generated datasets but will also be investigating spermatic chromatin in the context of Ctcf mutant mice at Rowan University as this project matures.

Memberships

American Society for Biochemistry and Molecular Biology
Genetics Society of America
Sigma Xi

Recent Publications

  1. Sharma FNU, Conine CC, Shea JM, Carone BR, Belleannee C, Li X, Bing XY, Fauquier L, Chen Po-shen, Gu Weifeng, Fazzio TG, Sullivan R, Mello CC, Garber M, Rando OJ Paternal diet alters tRNA fragment levels throughout the male reproductive tract in mammals Science. 2015 Dec 31. PMID: 26721685
  2. Carone BR, Hung JH, Hainer SJ, Min-Te Chou, Carone DM, Weng Z, Fazzio TG, Rando OJ High Resolution Mapping of Chromatin Packaging in mouse ES cells and sperm Dev Cell. 2014 Jul 14;30(1):11-22 PMID: 24998598
  3. Carone BR, Tao Xu, Kenan Murphy and Marinus MG High Frequency Mutation to Antibiotic Resistance in Enterohemorrhagic Escherichia coli O157:H7 Mutation Research - Fundamental & Molecular Mechanisms of Mutagenesis. 2014 Jan;759:1-8.  PMID: 24361397
  4. Carone BR*, Fauquier L*, Habib N*, Shea JM*, Hart CE, Li R, Bock C, Li C, Gu H, Zamore PD, Meissner A, Weng Z, Hofmann HA, Friedman N, Rando OJ  Paternally induced transgenerational environmental reprogramming of metabolic gene expression in mammals. Cell. 2010 Dec 23;143(7):1084-96. PMID: 21183072

 

One Medical Center Drive
Stratford, New Jersey 08084-1501
Technical contact for the web site
Privacy Statement